A Soybean Seed Urease-Null Produces Urease in Cell Culture
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چکیده
منابع مشابه
Soybean leaf urease: a seed enzyme?
The soybean (Glycine max L. [Merrill]) var Itachi has 0.2 to 0.3% the urease activity found in developing embryos of a normal line, Prize. The hydroxyurea sensitivity and pH preference of this basal seed urease indicate that it represents a unique enzyme rather than an unusually low level of the normal seed urease. Itachi's seed urease is less sensitive to hydroxyurea inhibition (65-80% inhibit...
متن کاملComparisons of soybean urease isolated from seed and tissue culture.
Urease was purified 500-fold to electrophoretic homogeneity from ground, dry soybeans. Sodium dodecyl sulfate-acrylamide gel electrophoresis indicates a subunit size of 93,500 daltons which is identical with that of jack bean urease. In solutions of high ionic strength, there exists a single urease species (species 1) with a size of about 480,000 daltons based on agarose column chromatography a...
متن کاملUrease-null and hydrogenase-null phenotypes of a phylloplane bacterium reveal altered nickel metabolism in two soybean mutants.
Mutation at either of two genetic loci (Eu2 or Eu3) in soybean (Glycine max [L.] Merr.) results in a pleiotropic elimination of the activity of both major urease isozymes. Surprisingly, the phenotype of a phylloplane bacterium, Methylobacterium mesophilicum, living on the leaves of eu2/eu2 or eu3-e1/eu3-e1 mutants is also affected by these plant mutations. The bacteria isolated from leaves of t...
متن کاملSoybean Seed Treatment with Nickel Improves Biological Nitrogen Fixation and Urease Activity
Nickel (Ni) is an essential micronutrient required for plants’ metabolism due to its role as a structural component of urease and hydrogenase, which in turn perform nitrogen (N) metabolism in many legume species. Seed treatment with cobalt, molybdenum and Bradyrhizobium strains has been widely practiced to improve crops. Additionally, seed treatment together with Ni fertilization of soybean mig...
متن کاملSoybean Urease: Over a Hundred Years of Knowledge
Ureases are metalloproteins responsible for the one step hydrolysis of urea into ammonia and carbamate [1], the later then rapidly and spontaneously decomposes to form carbon di‐ oxide and a second molecule of ammonia [2]. Plant ureases hold a special place in science history, participating on some important landmarks of biochemistry. For instance, it was by the analysis of Canavalia ensiformis...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1982
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.69.5.1233